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Effects of interleukin-12 gene polymorphism on response to hepatitis B vaccination among hemodialysis Egyptian patients

The Egyptian Journal of Internal Medicine volume 31, pages 804–812 (2019)Cite this article

Abstract

Background

Hepatitis B virus (HBV) infection is a major health problem among hemodialysis (HD) patients. Interleukin (IL)-12 gene polymorphisms may be associated with immune response variability to recombinant HBV vaccines. The aim was to determine the correlation between IL-12 gene polymorphism and hepatitis B surface-antibody (HBs-Ab) titer in response to HBV vaccine among HD Egyptian patients.

Patients and methods

Seventy patients receiving long-term HD and 20 age-matched and sex-matched healthy controls were enrolled. All participants were non-HBV vaccinated and seronegative for HBV and HIV. Recombinant HBV vaccine was given (three-dose scheduled). Thereafter, HBs-Ab titer and IL-12 gene polymorphism were evaluated 8 weeks after the last vaccination dose.

Results

There was no response (HBs-Ab<10 μIU/ml) in 20% of HD patients and 10% of the controls. HBs-Ab titers showed no significant correlation with duration of HD, BMI, serum albumin, hemoglobin, leucocytic count, parathyroid hormone level, or IL-12 gene polymorphism. Responders to vaccination had significantly lower transferrin saturation and significantly higher levels of urea reduction ratio, Kt/V and lymphopenia. IL-12B genotype frequency was as follows: AA (58.3 vs. 55.6%), AC (37.5%) and CC (4.2 vs. 0%) in responders of either HD or control participants, respectively (P>0.05 for all).

Conclusion

There was no significant association between IL-12B gene polymorphism and HBs-Ab response in Egyptian HD patients. In HD patients, lymphocytopnea, diabetes mellitus (DM), high transferrin saturation and inefficient HD were associated with HBV vaccine hyporesponsiveness.

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Authors and Affiliations

  1. Internal Medicine, Gastroenterology, Hepatology and Endoscopy Unit, Faculty of Medicine, Mansoura University, Ahmad Maher Street, 6th October Street, Mansoura, 35511, Egypt

    Sawsan M. Abdel-Moniem & Mohammed A. Mohammed MD

  2. Clinical Pathology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Ziyad M. Essam El-Deen

  3. Nephrology Department, Mansoura International Hospital, Mansoura, Egypt

    Hanan M. Mostafa

Authors
  1. Sawsan M. Abdel-Moniem
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  2. Mohammed A. Mohammed MD
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  3. Ziyad M. Essam El-Deen
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  4. Hanan M. Mostafa
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Correspondence to Mohammed A. Mohammed MD.

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Abdel-Moniem, S.M., Mohammed, M.A., Essam El-Deen, Z.M. et al. Effects of interleukin-12 gene polymorphism on response to hepatitis B vaccination among hemodialysis Egyptian patients. Egypt J Intern Med 31, 804–812 (2019). https://doi.org/10.4103/ejim.ejim_85_19

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  • DOI: https://doi.org/10.4103/ejim.ejim_85_19

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