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The influence of single-nucleotide polymorphisms of interleukin-1β -511 and +3954 on the susceptibility to Hashimoto’s thyroiditis in Egyptian women: immune-endocrine interactions

The Egyptian Journal of Internal Medicine volume 31, pages 14–21 (2019)Cite this article

Abstract

Background

Hashimoto’s thyroiditis (HT) is a T-cell-mediated autoimmune disease. Cytokines play a crucial role in modulating immune responses that affect the balance between maintenance of self-tolerance and initiation of autoimmunity. Thus, we aimed to explore the possible associations of interleukin (IL-1β) –511C/T (rs 16944) and IL-1β +3954C/T (rs1143634) gene polymorphisms with susceptibility of HT, and to clarify the impact of these polymorphisms on thyroid function of Egyptian women.

Patients and methods

Polymorphisms of the IL-1β –511 and IL-1β +3954 genes were assessed in a case–control study comprising 110 HT patients and 90 controls. Genetic variants were genotyped by multiplex PCR. Polymorphisms of the IL-1β were studied by PCR-restriction fragment length polymorphism analysis.

Results

Our results revealed that the IL-1β –511 CT genotype distribution was significantly higher in HT patients than in controls. With regard to IL-1β +3954 gene polymorphisms, our results showed that there was a nonsignificant difference between the control and HT groups. Women carrying TT and CT genotype of IL-1β –511 had significantly higher values of C-reactive protein, thyroid-stimulating hormone, anti-thyroid peroxidase, and anti-thyroglobulin. As regards the IL-1β serum level, women carrying TT and TC genotype of IL-1β –511 had significantly higher values compared with patients carrying the CC genotype. Moreover, anti-thyroglobulin and free thyroxine were the only independently correlated factors with IL-1β serum level by linear regression analysis.

Conclusion

CT genotype distribution was significantly higher in patients with HT than in controls with regard to IL-1β –511 (C>T) gene polymorphisms.

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Authors and Affiliations

  1. Department of Internal Medicine, Faculty of Medicine, Zagazig University, 44519, Zagazig, Egypt

    Nearmeen M. Rashad MD, Mayada M. Mousa & Azza H. Abd El-Fatah

  2. Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

    Manar H. Soliman

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  1. Nearmeen M. Rashad MD
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  2. Manar H. Soliman
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  3. Mayada M. Mousa
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  4. Azza H. Abd El-Fatah
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Correspondence to Nearmeen M. Rashad MD.

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Rashad, N.M., Soliman, M.H., Mousa, M.M. et al. The influence of single-nucleotide polymorphisms of interleukin-1β -511 and +3954 on the susceptibility to Hashimoto’s thyroiditis in Egyptian women: immune-endocrine interactions. Egypt J Intern Med 31, 14–21 (2019). https://doi.org/10.4103/ejim.ejim_69_18

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