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CD4 cell counts, complete blood picture and lipid profile in HIV infected and AIDS patients in a specific populace from South India
The Egyptian Journal of Internal Medicine volume 29, pages 151–163 (2017)
Abstract
Context
AIDS is caused by a retrovirus known as HIV which breaks down the body’s immune system leaving the patient vulnerable to a host of life-threatening opportunistic infections, neurological disorders, or unusual malignancies. According to estimates by WHO and UNAIDS, 35 million people were living with HIV globally at the end of the year 2013. The first AIDS case in India was detected in the year 1986. Seldom studies have been conducted correlating these parameters in the Indian population.
Aim
The present study was carried out to evaluate the CD4 cell counts, complete blood picture, and lipid profile in HIV-infected patients and those with AIDS and correlate these parameters with those obtained in the sero-negative controls.
Materials and methods
This was a cross-sectional, hospital-based study. The included participants were divided into three groups: group A consisted of 500 patients who were without any systemic illness as healthy controls, group B consisted of 500 patients who were diagnosed as having HIV infection, and group C consisted of 500 patients diagnosed as having AIDS depending on their CD4 cell counts. Permission from the Ethical Committee of the Institution as well as Superintendent of Government Hospital was taken to conduct the study. Evaluation of CD4 cell counts in patients with HIV infection and AIDS was done using CyFlow counter, whereas complete blood picture, hemoglobin (Hb), packed cell volume, red blood cell and white blood cells (WBCs) and platelet counts, were analyzed using Sysmex XP 100, a compact, fully automated analyzer. Lipid profile was evaluated using an automated analyzer, Erba EM 360 powered by a diffraction grating photometer.
Results
The results were found to be statistically significant, with the P-value being less than 0.001, for the CD4 cell counts, Hb, WBCs, and platelet counts. The levels of total cholesterol and low-density lipoproteins (LDLs) were significantly decreased whereas triglycerides and very LDLs were significantly increased in patients with AIDS when compared with the control group and patients with HIV infection.
Conclusion
CD4 cell counts, Hb, WBCs and platelet counts as well as total cholesterol, LDLs, triglycerides and very LDLs were significantly altered in patients with HIV infection and those with AIDS when compared with the controls.
References
Khiangte L, Vidyabati RK, Singh MK, Devi SB, Singh TR, Singh WG. A study of serum lipid profile in human immunodeficiency virus (HIV) infected patients. J Indian Acad Clin Med 2007; 8:307–311.
Ananthanarayan R, Jayaram Paniker CK. Human immunodeficiency virus: AIDS. Ananthanarayan and Paniker’s textbook of microbiology. 7th ed. New Delhi: Universities Press; 2007.
Abbas AK. Diseases of immunity. Robbins & Cotran pathologic basis of disease. 7th ed. Philadelphia, PA: Elsevier; 2005.
HIV sentinels surveillance: 2010-11. A Technical Brief. National AIDS Control Organization (NACO).
Fauci AS, Lane HC. Human immunodeficiency disease (HIV): AIDS and related disorders. In: Braunwald E, Fauci AS, Kasper DL, Hauser SL, Longo DL, Jameson JL, editors. Harrison’s principle of internal medicine. 15th ed. New York: Tata McGraw-Hill Medical Publishing Division; 2001.
Abhinandan HB, Jain SK, Nyati A, Kumar R, Jain M, Bhuria J, et al. Cutaneous manifestations of HIV-infection in relation with CD4 cell counts in Hadoti Region. J Evol Med Dent Sci 2013; 2:7003–7014.
HIV Surveillance. 2012. A Technical Brief. National Agency for the Control of AIDS.
HIV Sentinel Surveillance 2012-13: A Technical Brief. National AIDS Control Organization (NACO).
Sharma G, Pai MK, Nagpal A. Prevalence of oral manifestations and their association with CD4/CD8 ratio and HIV viral load in South India. Int J Dent 2011; 2011:964278.
Kiran K, Shetty S. Oral and periodontal manifestations among HIV population in Southern India. Int J Basic Applied Med Sci 2013; 3:184–189.
Coyle TE. Hematologic complications of human immunodeficiency virus infection and the acquired immunodeficiency syndrome. Med Clin North Am 1997; 81:449–470.
Pasupathi P, Manivannan P, Manivannan U, Mathiyalagan D. Thyroid function, cardiac risk assessment profile and hematological changes during HIV infection and AIDS patients. J Medi 2010; 11:131–136.
Molinari JA, Glick M. Infectious diseases. In: Greenberg MS, Glick M, editors. Burket’s oral medicine, diagnosis and treatment. 10th ed. Ontario: BC Decker, Hamilton; 2003.
Adurogbangba MI, Aderinokun GA, Odaibo GN, Olaleye OD, Lawoyin TO. Oro-facial lesions and CD4 counts associated with HIV/AIDS in an adult population in Oyo State, Nigeria. Oral Dis 2004; 10:319–326.
Pranitha SS, Kulkarni MH. Hematological changes in HIV infection with correlation to CD4 cell count. Aust Med J 2012; 5:157–162.
Chanarat N, Chanarat P, Viratsethasin K, Suttajit M, Chiewsilp D. Biochemical and hematological manifestations of HIV/AIDS in Chiang Mai, Thailand. Southeast Asian J Trop Med Public Health 2001; 32:500–503.
Ghate MV, Mehendale SM, Mahajan BA, Yadav R, Brahme RG, Divekar AD, et al. Relationship between clinical conditions and CD4 counts in HIV-infected persons in Pune, Maharashtra, India. Natl Med J India 2000; 13:183–187.
Srirangaraj S, Venkatesha D. Absolute lymphocyte count as a surrogate marker for CD4 counts after six months of HAART initiation in a resource limited setting in India. Indian J Med Res 2012; 135:895–900.
Angelo ALD, Angelo CD, Torres AJL, Ramos AMC, Lima M, Netto EM, et al. Evaluating total lymphocyte counts as a substitute for CD4 counts in the follow-up of AIDS patients. Braz J Infect Dis 2007; 11:466–470.
Tiwari BR, Ghimire P, Malla S. Study on CD4 cell responses in HIV. Nepal Med Coll J 2008; 10:45–47.
Mbanya D, Assah F, Ndembi N, Kaptue L. Monitoring anti-retroviral therapy in HIV/AIDS patients in resource-limited settings: CD4 counts or total lymphocyte counts?. Int J Infect Dis 2007; 11:157–160.
Sen S, Vyas A, Sanghi S, Shanmuganandan K, Gupta RM, Kapila K, et al. Correlation of CD4+ T cell count with total lymphocyte count, hemoglobin and erythrocyte sedimentation rate levelsinhuman immunodeficiency virus type-l disease. Med J Armed Forces India 2011; 67:15–20.
Edathodu J, Ali B, Alrajhi AA. CD4 validation for the World Health Organization classification and clinical staging of HIV/AIDS in a developing country. Int J Infect Dis 2009; 13:243–246.
Pasupathi P, Bakthavathsalam G, Saravanan G, Devaraj A. Changes in CD4 cell count, lipid profile and liver enzymes in HIV infection and AIDS patients. J Appl Biomed 2008; 6:139–145.
Sharma YK, Sawhney MPS, Bhakuni DS, Gera V. Oro-cutaneous manifestations as markers of disease progression in HIV infection in Indian setting. Med J Armed Forces India 2004; 60:239–243.
Pasupathi P, Ramachandran T, Sindhu P, Saravanan G, Bakthavathsalam G. Enhanced oxidative stress markers and antioxidant imbalance in HIV infection and AIDS patients. J Sci Res 2009; 1:370–380.
De Santis GC, Brunetta DM, Vilar FC, Brandão RA, de Albernaz Muniz RZ, de Lima GM, et al. Hematological abnormalities in HIV-infected patients. Int J Infect Dis 2011; 15:e808–e811.
Tagoe DNA, Asantewaa E. Profiling hematological changes in HIV patients attending fevers clinic at the Central Regional Hospital in Cape Coast, Ghana: a case–control study. Arch Appl Sci Res 2011; 3:326–331.
Treacy M, Lai L, Costello C, Clark A. Peripheral blood and bone marrow abnormalities in patients with HIV related disease. Br J Hematol 1987; 65:289–294.
Kaloutsi V, Kohlmeyer U, Maschek H, Nafe R, Choritz H, Amor A, et al. Comparison of bone marrow and hematologic findings in patients with human immunodeficiency virus infection and those with myelo-dysplastic syndromes and infectious diseases. Am J Clin Pathol 1994; 101:123–129.
Erhabor O, Ejele OA, Nwauche CA, Buseri FI. Some hematological parameters in human immunodeficiency virus (HIV) infected Africans: the Nigerian perspective. Niger J Med 2005; 14:33–38.
Jost J, Täuber MG, Lüthy R, Siegenthaler WL. HIV-associated thrombocytopenia. Schweiz Med Wochenschr 1988; 118:206–212.
Costello C. Hematological abnormalities in human immunodeficiency virus (HIV) disease. J Clin Pathol 1988; 41:711–715.
Karcher DS, Frost AR. The bone marrow in human immunodeficiency virus (HIV)-related disease: morphology and clinical correlation. Am J Clin Pathol 1991; 95:63–71.
Akinbami A, Oshinaike O, Adeyemo T. Hematologic abnormalities in treatment-naive HIV patients, Lagos, Nigeria. Infect Dis 2010; 3:45–49.
Mir N, Costello C, Luckit J, Lindley R. HIV-disease and bone marrow changes: a study of 60 cases. Eur J Haematol 1989; 42:339–343.
Walsh CM, Nardi MA, Karpatkin S. On the mechanism of thrombocytopenic purpura in sexually active homosexual men. N Engl J Med 1984; 311:635–639.
Karpatkin S. Immunologic thrombocytopenic purpura in patients at risk for AIDS. Blood Rev 1987; 1:119–125.
Harbol AW, Liesveld JL, Simpson-Haidaris PJ, Abboud CN. Mechanisms of cytopenia in human immunodeficiency virus infection. Blood Rev 1994; 8:241–250.
Nery MW, Martelli CMT, Turchi MD. Dyslipidemia in AIDS patients on highly active anti-retroviral therapy. Braz J Infect Dis 2011; 15:151–155.
Anastos K, Lu D, Shi Q, Tien PC, Kaplan RC, Hessol NA, et al. Association of serum lipid levels with HIV sero-status, specific anti-retroviral agents and treatment regimens. J Acquir Immune Defic Syndr 2007; 45:34–42.
Adewole OO, Eze S, Betiku Y, Anteyi E, Wada I, Ajuwon Z, Erhabor G. Lipid profile in HIV/AIDS patients in Nigeria. Afr Health Sci 2010; 10:144–149.
Grunfeld C. Dyslipidemia and its treatment in HIV infection. Top HIV Med 2010; 18:112–118.
Shor-Posner G, Basit A, Lu Y, Cabrejos C, Chang J, Fletcher M, et al. Hypocholesterolemia is associated with immune dysfunction in early human immunodeficiency virus-1 infection. Am J Med 1993; 94:515–519.
Souza SJ, Luzia LA, Santos SS, Helen P. Lipid profile of HIV infected patients in relation to anti-retroviral therapy: a review. Rev Assoc Med Bras 2013; 59:186–198.
Agbelusi GA, Wright AA. Oral lesions as indicators of HIV infection among routine dental patients in Lagos, Nigeria. Oral Dis 2005; 11:370–373.
Young J, Weber R, Rickenbach M, Furrer H, Bernasconi E, Hirschel B, et al. Lipid profiles for anti-retroviral naive patients starting PI- and NN-RTI based therapy in the Swiss HIV cohort study. Antivir Ther 2005; 10:585–591.
Crook M. The basis and management of metabolic abnormalities associated with cardiovascular risk in human immunodeficiency virus infection and its treatment. Ann Clin Biochem 2007; 44:219–231.
Pynka ML, Bauder D, Pynka S. Boron-Kaizmarsk. HIV/AIDS Rev 2004; 2:35–38.
Iffen TS, Efobi H, Usoro CAO, Udonwa NE. Lipid profile of HIV positive patients attending University of Calabar Teaching Hospital, Nigeria. World J Med Sci 2010; 5:89–93.
El-Sadir WM. Effects of HIV disease on lipid, glucose and insulin levels: results from a large anti-retroviral naive cohort. HIV Med 2005; 6:114–121.
Akpa MR, Agomouh DI, Alasia DD. Lipid profile of healthy adult Nigerians in Port Harcourt, Nigeria. Niger J Med 2006; 15:137–140.
Rogowska-Szadkowska D, Borzuchowska A. The levels of triglycerides, total cholesterol and HDL cholesterol in various stages of human immunodeficiency virus (HIV) infection. Pol Arch Med Wewn 1999; 101:145–150.
Ducobu J, Payen MC. Lipids and AIDS. Rev Med Brux 2000; 21:11–17.
Crook MA, Mir N. Abnormal lipids and the acquired immune deficiency syndrome is there a problem and what should we do about it. Int J STD AIDS 1999; 10:353–356.
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Ratnam, M.V.R., Nayyar, A.S., Kalivara, P.B. et al. CD4 cell counts, complete blood picture and lipid profile in HIV infected and AIDS patients in a specific populace from South India. Egypt J Intern Med 29, 151–163 (2017). https://doi.org/10.4103/ejim.ejim_59_17
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DOI: https://doi.org/10.4103/ejim.ejim_59_17