Table 4 The curve of receiver operating characteristic (ROC) of the studied indices for optimum cutoff points in diagnosing thalassemia subtypes (compared to healthy controls)
Indices | Cutoff | AUC | 95% CI | Sensitivity (%) | Specificity (%) | PPV (%) | NPV (%) | Youden index (%) | p-value |
|---|---|---|---|---|---|---|---|---|---|
Thalassemia minor | |||||||||
 RDWI | > 273.91 | 0.628 | (0.507, 0.738) | 34.78 | 100 | 100 | 76.9 | 34.78 | 0.125 |
 ML_I | ≤ 14.62 | 0.653 | (0.532, 0.760) | 56.52 | 80 | 54.5 | 78.4 | 36.52 | 0.049 |
 GK | > 81.8 | 0.540 | (0.419, 0.657) | 43.48 | 92 | 71.4 | 78 | 35.48 | 0.659 |
 EF | ≤ 5.6 | 0.617 | (0.496, 0.728) | 47.83 | 84 | 57.9 | 77.8 | 31.83 | 0.161 |
 SL | ≤ 1438.5 | 0.814 | (0.706, 0.895) | 69.57 | 100 | 100 | 87.7 | 69.57 | < 0.001* |
 SI | ≤ 4.27 | 0.641 | (0.520, 0.750) | 39.13 | 100 | 100 | 78.1 | 39.13 | 0.071 |
 RI | > 2.89 | 0.760 | (0.646, 0.853) | 69.57 | 86 | 69.6 | 86 | 55.57 | 0.001* |
 EI | ≤ 20 | 0.711 | (0.593, 0.811) | 47.83 | 94 | 78.6 | 79.7 | 41.83 | 0.004* |
 Sir_I | ≤ 33.9 | 0.693 | (0.575, 0.796) | 60.87 | 88 | 70 | 83 | 48.87 | 0.019* |
 HH | > 22.38 | 0.764 | (0.650, 0.855) | 69.57 | 98 | 94.1 | 87.5 | 67.57 | 0.001* |
Thalassemia intermedia | |||||||||
 RDWI | > 273.91 | 0.685 | (0.570, 0.786) | 39.29 | 100 | 100 | 74.6 | 39.29 | 0.009* |
 ML_I | ≤ 14.57 | 0.562 | (0.445, 0.674) | 50 | 82 | 60.9 | 74.5 | 32 | 0.445 |
 GK | > 88.2 | 0.511 | (0.395, 0.626) | 28.57 | 100 | 100 | 71.4 | 28.57 | 0.893 |
 EF | > 15 | 0.585 | (0.468, 0.696) | 53.57 | 72 | 51.7 | 73.5 | 25.57 | 0.235 |
 SL | ≤ 1401.7 | 1 | (0.954, 1) | 100 | 100 | 100 | 100 | 100 | < 0.001* |
 SI | > 4.42 | 0.519 | (0.402, 0.633) | 75 | 2 | 30 | 12.5 | 23 | 0.814 |
 RI | > 3.04 | 0.974 | (0.910, 0.997) | 92.86 | 100 | 100 | 96.2 | 92.86 | < 0.001* |
 EI | ≤ 23 | 0.753 | (0.642, 0.844) | 60.71 | 90 | 77.3 | 80.4 | 50.71 | 0.001* |
 Sir_I | ≤ 34.7 | 0.804 | (0.698, 0.885) | 67.86 | 86 | 73.1 | 82.7 | 53.86 | < 0.001* |
 HH | > 22.52 | 0.897 | (0.807, 0.954) | 82.14 | 100 | 100 | 90.9 | 82.14 | < 0.001* |
Thalassemia major | |||||||||
 RDWI | > 273.91 | 0.748 | (0.659, 0.824) | 43.94 | 100 | 100 | 57.5 | 43.94 | < 0.001* |
 ML_I | ≤ 14.53 | 0.623 | (0.528, 0.711) | 53.03 | 84 | 81.4 | 57.5 | 37.03 | 0.023* |
 GK | > 82.12 | 0.771 | (0.683, 0.844) | 56.06 | 94 | 92.5 | 61.8 | 50.06 | < 0.001* |
 EF | > 17.7 | 0.776 | (0.689, 0.848) | 56.06 | 88 | 86 | 60.3 | 44.06 | < 0.001* |
 SL | ≤ 1631.2 | 0.993 | (0.956, 1) | 93.94 | 100 | 100 | 92.6 | 93.94 | < 0.001* |
 SI | > 6.24 | 0.529 | (0.434, 0.622) | 36.36 | 94 | 88.9 | 52.8 | 30.36 | 0.593 |
 RI | > 3.04 | 0.972 | (0.923, 0.994) | 90.91 | 100 | 100 | 89.3 | 90.91 | < 0.001* |
 EI | ≤ 24 | 0.722 | (0.631, 0.801) | 69.7 | 88 | 88.5 | 68.7 | 57.7 | < 0.001* |
 Sir_I | ≤ 37.2 | 0.646 | (0.551, 0.732) | 65.15 | 68 | 72.9 | 59.6 | 33.15 | 0.005* |
 HH | > 22.52 | 0.935 | (0.874, 0.973) | 87.88 | 100 | 100 | 86.2 | 87.88 | < 0.001* |