Gene polymorphism | Primer sequence (5′ to 3′) | Melting temp. | Amplicon length |
---|---|---|---|
PTX3 (rs2305619) A/G | Forward outer primer ACTCAGCTCACTTGAGAGTCTCCTCCCG | 66 °C |  |
Reverse outer primer CGCCGGTTATGTAGCTCAAAACAGTCAA | 66 °C | 439 bp | |
Forward inner primer (A allele) CCATCCCACTGAGGACCGTAAGTGCA | 68 °C | 255 bp | |
Reverse inner primer (G allele) AGTCAGGGTTAGCAGAGAAACAGTTAACGC | 68 °C | 230 bp | |
PVT1 (rs2648875) G/A | Forward outer primer GGTTACATGTGTAAGCTCATCCAATCCC | 64 °C |  |
Reverse outer primer TAGTAGAGACGGGGTTTCACCATGTTAGC | 64 °C | 390 bp | |
Forward inner primer (G allele) GTAAGTCATTGGTCTTTGCAGGTGATGA | 66 °C | 239 bp | |
Reverse inner primer (A allele) TGACTTTCTTTTTTCCTGCCTCACACTC | 66 °C | 207 bp |