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Table 1 Designed primer sequences used in Tetra-Arms PCR genotyping

From: Association between PTX3 and PVT1 genetic polymorphisms and the risk of diabetic kidney disease in type 2 diabetic patients

Gene polymorphism

Primer sequence (5′ to 3′)

Melting temp.

Amplicon length

PTX3 (rs2305619)

A/G

Forward outer primer

ACTCAGCTCACTTGAGAGTCTCCTCCCG

66 °C

 

Reverse outer primer

CGCCGGTTATGTAGCTCAAAACAGTCAA

66 °C

439 bp

Forward inner primer (A allele)

CCATCCCACTGAGGACCGTAAGTGCA

68 °C

255 bp

Reverse inner primer (G allele)

AGTCAGGGTTAGCAGAGAAACAGTTAACGC

68 °C

230 bp

PVT1 (rs2648875)

G/A

Forward outer primer

GGTTACATGTGTAAGCTCATCCAATCCC

64 °C

 

Reverse outer primer

TAGTAGAGACGGGGTTTCACCATGTTAGC

64 °C

390 bp

Forward inner primer (G allele)

GTAAGTCATTGGTCTTTGCAGGTGATGA

66 °C

239 bp

Reverse inner primer (A allele)

TGACTTTCTTTTTTCCTGCCTCACACTC

66 °C

207 bp